For the warming process is necessary to prepare the SafeBox container with liquid nitrogen in a tank and water at 37 ° C in the other tank within 15 cm of the cryocane. Then you have to carefully slide the plastic cover up, to make sure the capillary is completely immersed in liquid nitrogen and in less than a second inmerse it in the hot water tank. Finally, place the bracket SafeSpeed in a cutting system to cut it just above the heat sealing in the external wider and below the 1st mark and expel the sample.