The Vitrification process must initially prepare samples in special glazing medium and subsequently introduce in the support SafeSpeed Device between the 2nd and 3rd mark by the method of aspiration with the connector. Once satisfied that the sample is well placed in the holder, heat seal the fine capillary with SafeSealer accessory brand below 1. Finally, the support should be immersed in the sealing vessel SafeBox Liquid Nitrogen filling and slide protective case support