The composition of Safespeed media works effectively at room temperature, allowing proper exchange between water and cryoprotectants and preserving the cell membrane intact. The only step that must be carried out at 37°C is the first of the devitrification procedure.
Vitrification medium (up to 10 vitrifications):
- 2ml Basic solution
- 2ml Equilibration solution
- 2ml Vitrification solution
- Shelf life: 1 year
Warming media (up to 10 reheats):
- 2ml Thawing Solution
- 2ml Diluent solution
- 2ml washing solution
- Shelf life: 1 year
Quality of each batch:
- SAL 10-6 Sterility Test
- pHTest
- Entotoxin test by LAL Limulus Amebocyte Lysate
- Visual inspection during composition, dosage and labeling
- Test Mouse Embryo Assay (MEA)
IMPORTANT NOTE: SafeSpeed media have the CE Registration, therefore they can be acquired and used for final use. The CE registration document can be consulted in the “Documents” section of this website.